Western Blotting - Principle, Procedure and Applications
18.5 هزار بار بازدید -
4 سال پیش
-
This video explains about Western
This video explains about Western Blotting - Principle, Procedure and Applications.
Link to SDS-PAGE:
SDS PAGE - Principle of SDS PAGE and ...
Western blotting / Immunoblotting is an analytical technique used to detect a target protein in a sample (containing a complex mixture of proteins) by using a polyclonal or monoclonal antibody specific to that protein. The mixture of protein can be separated based on their size by poly acrylamide gel electrophoresis, The protein bands are transferred to blotting membrane, the most widely used membranes for western blotting are Nitrocellulose and Polyvinylidene fluoride (PVDF) membranes.
Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: Plotting Bacterial Growth Curve in Excel
Colony Forming Units: CFU: Colony Forming Unit & cfu/ml cal...
Serial Dilution Methods: Serial Dilution Methods & Calaculations
Copy Number Calculation for qPCR: Copy Number Calculation for qPCR
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: How to analyze Primer Sequence or Oli...
How to generate qPCR standard curve in excel and calculate PCR efficiency: How to generate qPCR standard curve i...
Real Time qPCR optimization, Calculating PCR Efficiency: Real Time qPCR optimization, Calcula...
Taqman Assay Vs SYBR Green Assay: Real Time PCR Assays- Taqman Assay Vs...
Resolving poor PCR efficiency: Resolving Poor PCR assay Efficiency i...
Calculating molecular weight of Unknown protein How to calculate molecular weight of ...
Designing qPCR Primers
Primer designing for real time PCR us...
Oligonucleotide Preparation & Purification Oligonucleotide Preparation and Purif...
How to check Oligo Concentration
How to check the Oligo concentration ...
Link to SDS-PAGE:
SDS PAGE - Principle of SDS PAGE and ...
Western blotting / Immunoblotting is an analytical technique used to detect a target protein in a sample (containing a complex mixture of proteins) by using a polyclonal or monoclonal antibody specific to that protein. The mixture of protein can be separated based on their size by poly acrylamide gel electrophoresis, The protein bands are transferred to blotting membrane, the most widely used membranes for western blotting are Nitrocellulose and Polyvinylidene fluoride (PVDF) membranes.
Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: Plotting Bacterial Growth Curve in Excel
Colony Forming Units: CFU: Colony Forming Unit & cfu/ml cal...
Serial Dilution Methods: Serial Dilution Methods & Calaculations
Copy Number Calculation for qPCR: Copy Number Calculation for qPCR
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: How to analyze Primer Sequence or Oli...
How to generate qPCR standard curve in excel and calculate PCR efficiency: How to generate qPCR standard curve i...
Real Time qPCR optimization, Calculating PCR Efficiency: Real Time qPCR optimization, Calcula...
Taqman Assay Vs SYBR Green Assay: Real Time PCR Assays- Taqman Assay Vs...
Resolving poor PCR efficiency: Resolving Poor PCR assay Efficiency i...
Calculating molecular weight of Unknown protein How to calculate molecular weight of ...
Designing qPCR Primers
Primer designing for real time PCR us...
Oligonucleotide Preparation & Purification Oligonucleotide Preparation and Purif...
How to check Oligo Concentration
How to check the Oligo concentration ...
4 سال پیش
در تاریخ 1399/08/02 منتشر شده
است.
18,552
بـار بازدید شده