Resolving Poor PCR assay Efficiency in Real Time PCR
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10 سال پیش
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Real time PCR efficiency is one of the critical factor, in real time PCR assay development and optimization. A 100% percent efficient PCR result indicates a well optimized assay development. Below which indicates the need to further optimize the reaction parameters, which includes, primer probe concentration, buffer optimization, etc. An accepted PCR assay efficiency ranges from 90% to 110%.
A standard curve can be generated and efficiency can be calculated.
Real time PCR efficiency is one of the critical factor, in real time PCR assay development and optimization. A 100% percent efficient PCR result indicates a well optimized assay development. Below which indicates the need to further optimize the reaction parameters, which includes, primer probe concentration, buffer optimization, etc. An accepted PCR assay efficiency ranges from 90% to 110%.
A standard curve can be generated and efficiency can be calculated.
10 سال پیش
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