SDS PAGE - Principle of SDS PAGE and Use of Buffer System for Separation of Proteins
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This video explains about SDS
This video explains about SDS PAGE - Principle of SDS PAGE and Use of Buffer System for Separation of Proteins.
SDS stands for Sodium Dodecyl Sulphate and PAGE stands for polyacrylamide gel electrophoresis.
SDS PAGE is a technique used for separating proteins with difference in molecular weights.SDS PAGE can provide information about molecular weight, purity of particular protein preparation. The advantage of this technique is that it is simple and reproducible.
SDS PAGE Principle
SDS PAGE works by sieving effect of the polyacrylamide gel, polyacrylamide gel can be prepared with wide range of pore size by varying arcylamide - bisacrylamide ratio.SDS an anionic detergent, which binds to the protein and imparts net negative charge to the proteins. 1.4grams of SDS binds per gram of protein.Mercaptoethanol assists the protein denaturation by reducing all disulfide bonds.Ammonium per sulfate acts as initiator for polymerization and TEMED (tetramethylethylenediamine) helps in polymerization.
When electric current is applied proteins move through the gel pores based on the molecular weight it gets separated.
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SDS stands for Sodium Dodecyl Sulphate and PAGE stands for polyacrylamide gel electrophoresis.
SDS PAGE is a technique used for separating proteins with difference in molecular weights.SDS PAGE can provide information about molecular weight, purity of particular protein preparation. The advantage of this technique is that it is simple and reproducible.
SDS PAGE Principle
SDS PAGE works by sieving effect of the polyacrylamide gel, polyacrylamide gel can be prepared with wide range of pore size by varying arcylamide - bisacrylamide ratio.SDS an anionic detergent, which binds to the protein and imparts net negative charge to the proteins. 1.4grams of SDS binds per gram of protein.Mercaptoethanol assists the protein denaturation by reducing all disulfide bonds.Ammonium per sulfate acts as initiator for polymerization and TEMED (tetramethylethylenediamine) helps in polymerization.
When electric current is applied proteins move through the gel pores based on the molecular weight it gets separated.
Related Videos:
---------------------------------------------------------------------------------------------------------------
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Colony Forming Units: CFU: Colony Forming Unit & cfu/ml cal...
Serial Dilution Methods: Serial Dilution Methods & Calaculations
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How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: How to analyze Primer Sequence or Oli...
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Primer designing for real time PCR us...
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4 سال پیش
در تاریخ 1399/07/20 منتشر شده
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