Primer designing for real time PCR using NCBI Primer Blast

This video explains how to design primers for real time pcr using primer blast.
NCBI's primer blast tool helps in designing primers and also allows to check the specificity of the designed primers.
Various options are available to improve the primer stringency.

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• #real_time_pcr
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• #melting_temparature
• #tm
• #annealing_temparature