Biosensor-Principle-Working-Application in Pharmacy-Biotechnology-Unit1- B Pharm 6 Sem-Lect.4-Part-2
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What is biosensors: are analytical devices which measure concentration of an analyte. in this biological material interact with an analyte this interaction produce some physical and detectable changes which is measured and converted into electrical signal by a transducer.
finally the electrical signal is amplified interpreted and displayed as an analyte concentration in the solution or preparation.
an analyte is a compound whose concentration is to be determined.
example of biosensor -enzyme based electrode, glucose biosensor
in enzyme based electrode -an enzyme can be combined with electrochemical sensor , the enzyme electrode is a combination of any electrochemical probe with a thin layer 10 to 200mm of immobilized enzyme . the enzyme located in the membrane produce such as hydrogen and oxygen , ammonium and carbon dioxide or ever other small molecules depending on enzymatic reaction .
enzyme electrode types amperometric enzyme electrode, potentiometric enzyme electrode, conductometric enzyme electrode.
1) urease enzyme based electrode is the example of of enzyme based electrode - in this urea is enzyme is mixed with polyacrylamide gel convert urea into ammonium ions, ammonium ions can be detected by Ion electrode , resulting potential recorded is proportional to concentration of urea into the sample solution.
2) in glucose biosensor - it is based on a thin layer of glucose oxidase on an oxygen electrode. glucose react with glucose oxidase to form gluconic acid and form reduced glucose oxidase. if the glucose concentration higher than more oxygen is consumed, if the glucose concentration lower then hydrogen peroxide concentration increased
so the consumption of oxygen for the production of hydrogen peroxide can be detected by platinum electrode and this can serve as a measure for glucose concentration.
#Pharmaceutical_Biotechnology
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What is biosensors: are analytical devices which measure concentration of an analyte. in this biological material interact with an analyte this interaction produce some physical and detectable changes which is measured and converted into electrical signal by a transducer.
finally the electrical signal is amplified interpreted and displayed as an analyte concentration in the solution or preparation.
an analyte is a compound whose concentration is to be determined.
example of biosensor -enzyme based electrode, glucose biosensor
in enzyme based electrode -an enzyme can be combined with electrochemical sensor , the enzyme electrode is a combination of any electrochemical probe with a thin layer 10 to 200mm of immobilized enzyme . the enzyme located in the membrane produce such as hydrogen and oxygen , ammonium and carbon dioxide or ever other small molecules depending on enzymatic reaction .
enzyme electrode types amperometric enzyme electrode, potentiometric enzyme electrode, conductometric enzyme electrode.
1) urease enzyme based electrode is the example of of enzyme based electrode - in this urea is enzyme is mixed with polyacrylamide gel convert urea into ammonium ions, ammonium ions can be detected by Ion electrode , resulting potential recorded is proportional to concentration of urea into the sample solution.
2) in glucose biosensor - it is based on a thin layer of glucose oxidase on an oxygen electrode. glucose react with glucose oxidase to form gluconic acid and form reduced glucose oxidase. if the glucose concentration higher than more oxygen is consumed, if the glucose concentration lower then hydrogen peroxide concentration increased
so the consumption of oxygen for the production of hydrogen peroxide can be detected by platinum electrode and this can serve as a measure for glucose concentration.
#Pharmaceutical_Biotechnology
---------------------------------------------------------------------------------------------------------------------------------------------------------------
My Telegram account: https://t.me/joinchat/UrMLkw_VPi5RMbO...
-----------------------------------------------------------------------------
My Facebook Page: https://www.facebook.com/MS-Sci-Acade...
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3 سال پیش
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