DNA Melting Temperature [Tm] - Factors affecting DNA melting Temperature
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This video explains about DNA
This video explains about DNA Melting Temperature [Tm] - Factors affecting DNA melting Temperature.
DNA melting Temperature: Tm Definition:
The Temperature of Melting (Tm) is defined as the temperature at which 50% of double stranded DNA is changed to single-standard DNA.
Factors affecting melting temperature:
There are three main factors that affects the Tm of DNA and these are
1. Nucleotide Composition
2. Length of the DNA
3. Salt Concentration
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DNA melting Temperature: Tm Definition:
The Temperature of Melting (Tm) is defined as the temperature at which 50% of double stranded DNA is changed to single-standard DNA.
Factors affecting melting temperature:
There are three main factors that affects the Tm of DNA and these are
1. Nucleotide Composition
2. Length of the DNA
3. Salt Concentration
Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: Plotting Bacterial Growth Curve in Excel
Colony Forming Units: CFU: Colony Forming Unit & cfu/ml cal...
Serial Dilution Methods: Serial Dilution Methods & Calaculations
Copy Number Calculation for qPCR: Copy Number Calculation for qPCR
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: How to analyze Primer Sequence or Oli...
How to generate qPCR standard curve in excel and calculate PCR efficiency: How to generate qPCR standard curve i...
Real Time qPCR optimization, Calculating PCR Efficiency: Real Time qPCR optimization, Calcula...
Taqman Assay Vs SYBR Green Assay: Real Time PCR Assays- Taqman Assay Vs...
Resolving poor PCR efficiency: Resolving Poor PCR assay Efficiency i...
Calculating molecular weight of Unknown protein How to calculate molecular weight of ...
Designing qPCR Primers
Primer designing for real time PCR us...
Oligonucleotide Preparation & Purification Oligonucleotide Preparation and Purif...
How to check Oligo Concentration
How to check the Oligo concentration ...
3 سال پیش
در تاریخ 1400/04/23 منتشر شده
است.
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بـار بازدید شده