DNA Melting Temperature [Tm] - Factors affecting DNA melting Temperature

This video explains about DNA Melting Temperature [Tm] - Factors affecting DNA melting Temperature.

DNA melting Temperature: Tm Definition:

The Temperature of Melting (Tm) is defined as the temperature at which 50% of double stranded DNA is changed to single-standard DNA.

Factors affecting melting temperature:
There are three main factors that affects the Tm of DNA and these are

1. Nucleotide Composition
2. Length of the DNA
3. Salt Concentration

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