GENE CLONING In Detail - Part 1 | CSIR

In this video I have discussed about what is gene cloning, what are the steps involved in gene cloning.
In molecular biology, cloning a gene means inserting the sequence of the gene into a plasmid, which is a small piece of circular DNA that can be "transfected"  into a bacterial strain, where the plasmid will replicate to many, many copies. The plasmid carries an antibiotic resistance gene, so when the bacteria are grown in the presence of the antibiotic, only those bacterial cells with the plasmid in them, can multiply.

This allows molecular biologists to easily propagate and grow vast amounts if the gene, and also to manipulate it in many ways, for example to introduce mutations to study its function, etc.

Please watch this video for more detail. If you like this video please like and share this video, and don’t forget to subscribe and click on bell icon for new videos.

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