DNA Cloning Part 5 - Linkers and Adaptors | DNA ligation | Recombinant DNA Technology | In Hindi
51.1 هزار بار بازدید -
5 سال پیش
-
linkers and adaptors in recombinant
linkers and adaptors in recombinant DNA technology- in Hindi
DNA ligation is one of the most crucial steps in the development of the DNA vector with the gene of interest. For ligating the gene of interest to the vector, we use DNA ligase and T4 ligase. However, in some conditions, it is possible that the gene of interest may have blunt ends instead of sticky ends after the treatment with the restriction endonucleases. The ligation of the blunt end fragment of the gene with the vector having sticky ends is difficult. In such condition, we use the linkers and adapters to generate the sticky ends.
for more details please watch this video
If you liked this video, please do like, share and subscribe. :)
Thanks for watching.
Gene Cloning technique Part -1 | Steps involved in gene cloning Explained | In Hindi Gene Cloning technique Part -1 | Step...
DNA Cloning | Part 2 - Enzymes used in Gene Cloning DNA Cloning | Part 2 - Enzymes used i...
DNA Cloning Part 3 - DNA Modifying Enzymes | Nucleases DNA Cloning Part 3 - DNA Modifying En...
DNA Cloning Part 4 - Restriction Endonucleases DNA Cloning Part 4 - Restriction Endo...
learn more:
Telegram: https://t.me/baayo_channel
Facebook Group: Facebook: baayo
Facebook Page: Facebook: BaaYo-377840676198895
Unacademy: https://unacademy.com/@artirani
LinkedIn Page: LinkedIn: baayo
LinkedIn Profile: LinkedIn: artirani
DNA ligation is one of the most crucial steps in the development of the DNA vector with the gene of interest. For ligating the gene of interest to the vector, we use DNA ligase and T4 ligase. However, in some conditions, it is possible that the gene of interest may have blunt ends instead of sticky ends after the treatment with the restriction endonucleases. The ligation of the blunt end fragment of the gene with the vector having sticky ends is difficult. In such condition, we use the linkers and adapters to generate the sticky ends.
for more details please watch this video
If you liked this video, please do like, share and subscribe. :)
Thanks for watching.
Gene Cloning technique Part -1 | Steps involved in gene cloning Explained | In Hindi Gene Cloning technique Part -1 | Step...
DNA Cloning | Part 2 - Enzymes used in Gene Cloning DNA Cloning | Part 2 - Enzymes used i...
DNA Cloning Part 3 - DNA Modifying Enzymes | Nucleases DNA Cloning Part 3 - DNA Modifying En...
DNA Cloning Part 4 - Restriction Endonucleases DNA Cloning Part 4 - Restriction Endo...
learn more:
Telegram: https://t.me/baayo_channel
Facebook Group: Facebook: baayo
Facebook Page: Facebook: BaaYo-377840676198895
Unacademy: https://unacademy.com/@artirani
LinkedIn Page: LinkedIn: baayo
LinkedIn Profile: LinkedIn: artirani
5 سال پیش
در تاریخ 1398/09/17 منتشر شده
است.
51,141
بـار بازدید شده