Separation of Amino acids by TLC - Amrita University

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TLC  or Thin layer chrmoatography can be utilized to identify the different amino acids .Separation occurs as each component, being different in chemical  and physical composition, interacts with the stationary and mobile phases to a different degree creating the individual bands on the plate.

Mixtures of aminoacids can be separated on chromatographic paper. The separated amino acids are visualized using solution of ninhydrin. Purple color develops upon reaction of amino acid with ninhydrin.

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