Urease Test - Amrita University

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To determine the ability of microorganisms to degrade urea by means of the enzyme urease.
Urea is a nitrogen containing compound that is produced during decarboxylation of the amino acid arginine during the urea cycle. Urea is highly soluble in water and is therefore an efficient way for the human body to expel excess nitrogen. This excess urea is then taken out of the body via the kidneys as a component of urine. Some bacteria produce the enzyme urease as part of its metabolism to break down urea to ammonia and carbon dioxide.

The urease test identifies those organisms that are capable of hydrolyzing urea to produce ammonia and carbon dioxide. It is primarily used to distinguish urease-positive Proteeae from other Enterobacteriaceae.

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