Malaria Parasite Test |# dengue fever | Harib Diagnostic Lab

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Malaria Parasite Test |# dengue fever...

TEST PROCEDURE

1.  Dispense 2 drop (20μl) of whole blood to the “S” well of the test cassette using the plastic dropper provided according to the figure.
2.  Add three drops of Sample Diluent to the “D” well after the specimen is added.
3.  Interpret test results at 15-30 minutes.

MALARIA PARASITE

Malaria is one of the most serious and complex health problems facing humanity. Malaria is considered sometimes fatal parasitic disease characterized by fever, chills and anemia, which can be transmitted from one human to another by the bite of infected Anopheles mosquitoes.

There are four kinds of malaria that can affect humans namely Plasmodium Falciparum, P. Vivax , P. Ovale, and P. Malariae, out of which P. Falciparum is most predominant followed by P. Vivax. In human, the parasite called sporozoites migrate to the liver where they mature and release another form, the merozoites.

Over two billion people live in malaria-affected areas in the tropics and sub-tropics and each year approximately 300 million infections occur, resulting in up to 3 million deaths according to a report from World Health Organization.

The definite diagnosis of Plasmodium Falciparum (Pf) malaria continues to be cased on clinical criteria supported by microscopic examination of whole blood.

The Malaria (Pf/Pv) Test is an immunochromatographic (rapid) test for the qualitative detection of antibodies of all isotopes (IgG, IgM, IgA) specific to Plasmodium falciparum and Plasmodium vivax simultaneously in human serum, plasma or whole blood. The Malaria Pf/Pv test contains a membrane strip, which is pre-coated with recombinant malaria P.f capture antigen on test band 1 region and with recombinant malaria P.v antigen on test band 2 region.  

INTERPRETATION OF RESULTS

Positive: Control line and at least one test line appear on the membrane. The appearing of T1 test line indicates a Pf. HRP-II positive result, the appearing of T2 test line indicates a P. vivax specific pLDH positive result, the appearing of both T1 and T2 test lines indicate both Pf. HRP-II and P. vivax specific pLDH positive result. The lower the antigen concentration is, the weaker the test line is.

Negative: Only the control band appears on the membrane. The absence of a test band indicates a negative result.

Note: It is normal to have a slightly lightened control band with very strong positive samples as long as it is distinctly visible.


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