ABTS [TEAC] - Trolox-Equivalent Antioxidant Capacity Assay - Principle, Advantages & Limitations
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This video explains about ABTS
This video explains about ABTS [TEAC] - Trolox-Equivalent Antioxidant Capacity Assay - Principle, Advantages & Limitations.
Miller and Rice-Evans were the first to report the ABTS•+ assay in 1993, The assay is also known as TEAC [Trolox-Equivalent Antioxidant Capacity].
Initial Method:
The generation of a radical cation (ABTS•+) was done by reacting metmyoglobin (Met-Myb) with hydrogen peroxide (H2O2) to produce hydroxyl radical (HO•).
Improved Method:
ABTS•+ radical generated by treating with Ammonium or Potassium persulfate in the ratio 1:0.5.
Oxidation of ABTS reaction is long process and requires 12-16hrs of incubation.
Principle:
The TEAC Test (Trolox-Equivalent Antioxidant Capacity) is based on the reaction with the colored and relatively persistent 2,2 ′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+ ) radical cation which has a strong absorption band at 734 nm.
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Miller and Rice-Evans were the first to report the ABTS•+ assay in 1993, The assay is also known as TEAC [Trolox-Equivalent Antioxidant Capacity].
Initial Method:
The generation of a radical cation (ABTS•+) was done by reacting metmyoglobin (Met-Myb) with hydrogen peroxide (H2O2) to produce hydroxyl radical (HO•).
Improved Method:
ABTS•+ radical generated by treating with Ammonium or Potassium persulfate in the ratio 1:0.5.
Oxidation of ABTS reaction is long process and requires 12-16hrs of incubation.
Principle:
The TEAC Test (Trolox-Equivalent Antioxidant Capacity) is based on the reaction with the colored and relatively persistent 2,2 ′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+ ) radical cation which has a strong absorption band at 734 nm.
DPPH Assay: Principle, Advantages & Limitations
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---------------------------------------------------------------------------------------------------------------
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Colony Forming Units: CFU: Colony Forming Unit & cfu/ml cal...
Serial Dilution Methods: Serial Dilution Methods & Calaculations
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How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: How to analyze Primer Sequence or Oli...
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Real Time qPCR optimization, Calculating PCR Efficiency: Real Time qPCR optimization, Calcula...
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3 سال پیش
در تاریخ 1400/05/03 منتشر شده
است.
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