Choosing a Cloning Technique

Choosing the correct cloning technique is a vital part of your cloning process. But sometimes, it can be hard to know where to start.

This video provides you with a guide on how to choose the proper technique for your experiment, including:
IN THIS VIDEO:
00:00
01:49 Vector plus 1 fragment
04:01 Vector plus 2 fragments
06:26 Vector plus 3-5+ fragments
07:28 Gibson Assembly
07:57 Gateway cloning
09:27 Golden Gate cloning

INSTRUCTOR: JULIA GEORGE-RAIZEN
Julia George-Raizen received from the University of Michigan in 1991 in what would now be called Molecular Immunology. She has done post-doctoral work and later research using mice, cell lines, yeast, and C. elegans.

Julia has a front-row seat to the development and evolution of all current molecular biology techniques, from PCR to CRISPR, and taught at several universities in the Philadelphia area, including St. Joseph's University and Bryn Mawr College. She now works as a consultant in science communication and education.

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