Primer design |in silico cloning | SnapGene | UCSC Genome browser

This video lecture explains
1. How to use UCSC genome browser to extract different regions of the gene of interest?
2. How to design the primers to amplify the region of interest using SnapGene?
3. How to design primers for cloning the insert ?
4. How to check the size of the PCR product flanked by the designed primers?
5. How to use Snapgene?
6. How to perform in silico cloning in snapgene?
7. How to select the restriction enzymes?
8. How to design primers with restriction enzyme site?

#cloning #biologylectures #genetics

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