TA and GC Cloning with SnapGene

TA cloning is used to clone PCR products. It takes advantage of the 3′ A overhangs added during amplification by Taq and some other polymerases. These sticky ends enable insertion into a linearized vector with complementary 3′ T overhangs. GC cloning is similar, and is based on the finding that Taq actually adds a mixture of 3′ A and 3′ G overhangs.
IN THIS VIDEO:
00:00
00:44 TA Cloning
03:16 GC Cloning

#SnapGene provides a simple interface for simulating TA and GC cloning. A variety of commercially available linearized TA and GC cloning vectors are embedded in the software.

Visit snapgene.com to learn more.
SnapGene Home: https://www.snapgene.com/
SnapGene Resources: https://www.snapgene.com/resources/
SnapGene Free Trial: https://www.snapgene.com/free-trial

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