Gateway cloning | How does Gateway cloning work? | What are the advantages of Gateway cloning? |
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this video would answer the
this video would answer the followig questions
How does Gateway cloning work?
What are the advantages of Gateway cloning?
What is Gateway recombination?
What is MultiSite gateway cloning?
What are the three methods that you can use to create a gateway entry clone?
What are the three main components you need to create an expression clone using Gateway cloning?
How do I clone a gateway?
What is entry clone?
The GATEWAY Cloning Technology is based on the site-specific recombination system used by phage l to integrate its DNA in the E. coli chromosome. Both organisms have specific recombination sites called attP in phage l site and attB in E. coli. The integration process (lysogeny) is catalyzed by 2 enzymes: the phage l encoded protein Int (Integrase) and the E. coli protein IHF (Integration Host Factor).
The process is reversible and the excision is again catalyzed Int and IHF in combination with the phage l protein Xis. The attL and attR sites surrounding the inserted phage DNA recombine site-specifically during the excision event to reform the attP site in phage l and the attB site in the E. coli chromosome.
How does Gateway cloning work?
What are the advantages of Gateway cloning?
What is Gateway recombination?
What is MultiSite gateway cloning?
What are the three methods that you can use to create a gateway entry clone?
What are the three main components you need to create an expression clone using Gateway cloning?
How do I clone a gateway?
What is entry clone?
The GATEWAY Cloning Technology is based on the site-specific recombination system used by phage l to integrate its DNA in the E. coli chromosome. Both organisms have specific recombination sites called attP in phage l site and attB in E. coli. The integration process (lysogeny) is catalyzed by 2 enzymes: the phage l encoded protein Int (Integrase) and the E. coli protein IHF (Integration Host Factor).
The process is reversible and the excision is again catalyzed Int and IHF in combination with the phage l protein Xis. The attL and attR sites surrounding the inserted phage DNA recombine site-specifically during the excision event to reform the attP site in phage l and the attB site in the E. coli chromosome.
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