Part 2: Overlap Extension PCR; Construct Preparation and DNA Transformation.
4.6 هزار بار بازدید -
2 سال پیش
-
This short video follows on
This short video follows on from Part 1: PCR primer design for Overlap Extension PCR.
PART1: PCR primer design.
PART2: DNA Gel electrophoresis and transformation of PCR product.
PART3: Confirmation of PCR construct uptake by the bacterial species under investigation.
In this video the PCR products generated from the primer sequences designed in the first video are shown run on an agarose gel and stained with SybrSafe. The video also covers an overview of method used to transform the PCR product into a bacterial species to "knockout" the gene under investigation.
PART1: PCR primer design.
PART2: DNA Gel electrophoresis and transformation of PCR product.
PART3: Confirmation of PCR construct uptake by the bacterial species under investigation.
In this video the PCR products generated from the primer sequences designed in the first video are shown run on an agarose gel and stained with SybrSafe. The video also covers an overview of method used to transform the PCR product into a bacterial species to "knockout" the gene under investigation.
2 سال پیش
در تاریخ 1401/06/03 منتشر شده
است.
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