Creating a Gene Knockout Mutant.
1 هزار بار بازدید -
2 سال پیش
-
This is a compilation of
This is a compilation of three short video parts, condensed into one longer video.
PART1: PCR primer design.
PART2: Gel electrophoresis and transformation of PCR product.
PART3: Confirmation of PCR construct uptake by the bacterial species under investigation.
The video covers all of the Primer design, DNA transformation and Homologous Recobination, gene knockout, DNA sequencing and antibiotic resistance.
Some of the methods outlined use Bioinformatics and PCR techniques for biotechnology.
I hope this is useful to anyone trying these sorts of things out for themselves.
PART1: PCR primer design.
PART2: Gel electrophoresis and transformation of PCR product.
PART3: Confirmation of PCR construct uptake by the bacterial species under investigation.
The video covers all of the Primer design, DNA transformation and Homologous Recobination, gene knockout, DNA sequencing and antibiotic resistance.
Some of the methods outlined use Bioinformatics and PCR techniques for biotechnology.
I hope this is useful to anyone trying these sorts of things out for themselves.
2 سال پیش
در تاریخ 1401/08/21 منتشر شده
است.
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