Fluorescent Western Blot video protocol

Fluorescent western blotting is ideal for multiplexing and quantitative analysis over a large dynamic range as it enables you to view multiple proteins simultaneously by using secondary antibodies conjugated to fluorescent labels. Watch our video protocol that takes you through key steps of fluorescent western blotting, including membrane blocking and staining with primary and secondary antibodies.

Timestamps
00:00 Introduction
00:19 What you will need
00:32 Blocking buffers
01:00 Incubation
01:36 Add primary antibody solution
01:55 Begin washing steps
03:14 Incubate the membrane
03:34 The membranes are ready for imaging

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