Direct and indirect flow cytometry video protocol

Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. Watch our flow cytometry video protocol that teaches you how to prepare and stain your cells for flow cytometry analysis.

View all our video protocols with written procedures at: http://www.abcam.com/tag/video-protocols

Connect with us
Visit us on: https://www.abcam.com​​  
Facebook: Facebook: ​​
Twitter: Twitter: abcam​​
Instagram: Instagram: abcamlive


Timestamps:
00:00 Introduction
00:13 What you will need
00:41Titration (optional)
01:00 Preparing a primary antibody solution
01:15 Re-suspending cells and measuring cell viability
01:48 Centrifugation
02:03 Blocking step
02:37 Staining with primary antibody
03:51 Secondary antibody step (optional)
04:55 Checking for live/dead cells
05:16 Storing the cell suspension