How to use Primer-Blast to create PCR primers

This video gives a worked example of how to use the NCBI Primer-BLAST tool to work out which primers you will need for PCR.

The link to the tool is here: https://www.ncbi.nlm.nih.gov/tools/pr...

The DNA sequence used is here:

ATCTCAAGATCGTGCTGCAGACCAAGTGATCATACGAGATCTCTCGAATTCATGCATCTTCCGAGTCCCACTGACAGCAACTTCTATCGGGTGAATTGCGACGAAGTAGGTGGCGAAGCCTTGGGTCGCGCAGTCTTTCCGTCGATTGTCGGGCGTCCACGTCGTCTGACCAGTCTGGTGCGCGAAACGTTCATCACCGGCTTAGACGCTCCGCGTGGTGTGGTCGATTCGGAGGATCTGGAGCTGAACATCTCTCGGTTAGGGATTCACGAGGACAGCCAGAATCGCTTTGCGGATCTCTCAGAAGCAGCCAATCGCGCACAAGCGGAACTGGAAGCTCAGGAACTGCAGCGCGATAGCCAGGATGCCGGAGGTTTTGGCCCTGAGGATCGTTGCGAAGTGGGCTATACAGGCGTACGCGTTACGCATGCGGTTGTGACCGTTCCGGCGTACTTCAACGATGCGCAACGCGCCTGGCTCGAGCACCACCACCACCACCACTGAGATCCATCGTGAGATGCAAA GCCCATTTACCGTACCACCATCGTGATGTCTGGA