Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning
1.1 هزار بار بازدید -
10 ماه پیش
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References/Resources:
References/Resources: Patreon: the_Crux
Playlist - Genetic Engineering: Genetic Engineering (Genes to Genomes)
Molecular Cloning with restriction enzymes can be very easy or very challenging. This video discusses the fundamental considerations of traditional restriction enzyme based molecular cloning. We discuss sticky and blunt ends, the process of blunting, and how all of this (among other things) can be used to understand the different levels of molecular cloning. Doesn’t matter if you are doing subcloning or a PCR based cloning, you will eventually find yourself in one of the levels.
Jump to your favorite section:
00:00 Cloning and Tetris
00:56 Sticky ends
01:18 Where do you cut?
02:29 Blunt ends
02:49 Cheating with Klenow
04:42 Level 1
07:04 Level 2
08:21 Level 3
11:57 Level 4
14:25 Level 5
Patreon Page: Patreon: the_Crux
Suggested videos:
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Yeast Cloning Vectors: Lambda Phage vector, Cosmid cloning v...
BACs/Fosmids: F plasmid, BACs, Fosmid cloning vecto...
Lambda/Cosmids: Lambda Phage vector, Cosmid cloning v...
Must watch playlists
All videos on Genetic Engineering: Genetic Engineering (Genes to Genomes)
All videos on DNA Replication: All about DNA Replication!
All videos on Transcription: All about Transcription!
All videos on Translation: All about Translation!
Consider supporting the growth of this channel by becoming a Patron: Patreon: the_Crux
I appreciate your support :)
Playlist - Genetic Engineering: Genetic Engineering (Genes to Genomes)
Molecular Cloning with restriction enzymes can be very easy or very challenging. This video discusses the fundamental considerations of traditional restriction enzyme based molecular cloning. We discuss sticky and blunt ends, the process of blunting, and how all of this (among other things) can be used to understand the different levels of molecular cloning. Doesn’t matter if you are doing subcloning or a PCR based cloning, you will eventually find yourself in one of the levels.
Jump to your favorite section:
00:00 Cloning and Tetris
00:56 Sticky ends
01:18 Where do you cut?
02:29 Blunt ends
02:49 Cheating with Klenow
04:42 Level 1
07:04 Level 2
08:21 Level 3
11:57 Level 4
14:25 Level 5
Patreon Page: Patreon: the_Crux
Suggested videos:
Plasmids (pUC19, pBR322, etc.): Plasmid and Phagemid cloning vector -...
Yeast Cloning Vectors: Lambda Phage vector, Cosmid cloning v...
BACs/Fosmids: F plasmid, BACs, Fosmid cloning vecto...
Lambda/Cosmids: Lambda Phage vector, Cosmid cloning v...
Must watch playlists
All videos on Genetic Engineering: Genetic Engineering (Genes to Genomes)
All videos on DNA Replication: All about DNA Replication!
All videos on Transcription: All about Transcription!
All videos on Translation: All about Translation!
Consider supporting the growth of this channel by becoming a Patron: Patreon: the_Crux
I appreciate your support :)
10 ماه پیش
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