Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes

References/Resources: www.patreon.com/the_Crux This video discusses restriction independent methyl-transferases like dam and dcm methylase - their biological function and how they affect restriction enzyme behavior. Apart from Dam and Dcm methyl-transferases, we discuss EcoK1, mcrABC and mrr endonuclease as well. All this is done to recognize the importance of understanding DNA methylases and their impact on molecular cloning - four examples are discussed. Jump to your favorite section: 00:00 Outline 00:41 Dam methylase 01:42 Dcm methylase 02:57 EcoKI methylase 04:21 Dam Dcm affect restriction enzymes 06:09 Dam Dcm affect transformation 07:02 Dam Dcm affect repetitive DNA 07:32 mcrABC and mrr endonuclease 09:30 Dam Dcm are not important Patreon Page: www.patreon.com/the_Crux Suggested videos: Restriction Enzymes:    • Restriction Enzymes in Molecular Cloning   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Do leave a LIKE if you find this content useful! COMMENT your questions, suggestions, and thoughts :) And I hope you have SUBSCRIBED because there is a lot more coming! :D I appreciate your support.

• #education
• #dna_methylation
• #molecular_cloning
• #genetic_engineering
• #restriction_enzymes
• #recombinant_dna_technology
• #dam_methylase
• #dcm_methylase
• #dna_methyltransferase
• #dna_methylase
• #mcr_endonuclease
• #restriction_independent_methylases
• #function_of_dam_methylase
• #dam_methylase_role_in_dna_replication_initiation
• #dcm_methylase_sensitivity
• #dam_methylase_block_restriction_enzymes
• #dam_methylase_sensitivity
• #methyl_sensitivity_of_restriction_enzymes
• #dcm_dam_methylation
• #methylated_dna