Introduction to the Western Blot

The western blot is one of the most commonly used techniques in scientific research. This video will provide an overview of the western blot along with its history. In general, the western blot is a technique in which proteins are transferred from a gel onto a membrane. The proteins are then incubated with antibodies which allow for them to be detected.

This video was made by McMaster University students Aniqa Chaudhry, Brooke D'Mello, Ibrahim Ilahi, Kunwar Karim, Mubariz Maqsood, Adnan Moinuddin, and Julia Zhu in collaboration with the Demystifying Research McMaster Program.

Episode #2: SDS-PAGE: Western Blot: Introduction to SDS-PAGE

Episode #3: Wet Transfer: Western Blot: How to do a Wet Transfer

Episode #4: Electroblotting: Western Blot: Introduction to Electro...

Episode #5: Blocking: Western Blot: Introduction to Blocking

Episode #6: Immunodetection: Western Blot: Introduction to Immunod...

Episode #7: Results & Analysis: Western Blot: Result Interpretation

This video is for general and educational purposes only.

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References

Burnette, W. (1981). “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry, 112(2), 195–203. https://doi.org/10.1016/0003-2697(81)...

GenHunter Coporation. (n.d.). Who really invented the Western Blot? GenHunter. https://www.genhunter.com/western-blo...

Kurien, B. T., & Scofield, R. H. (2015). Other Notable Protein Blotting Methods: A Brief Review. Methods in Molecular Biology, 487–503. https://doi.org/10.1007/978-1-4939-26...

Renart, J., Reiser, J., & Stark, G. R. (1979). Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National Academy of Sciences, 76(7), 3116–3120. https://doi.org/10.1073/pnas.76.7.3116

Sawyer, A. (2022, January 6). South, north, east and west-ern: the story of how the western blot came into being. BioTechniques. https://www.biotechniques.com/biochem...

Towbin, H., Staehelin, T., & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, 76(9), 4350–4354. https://doi.org/10.1073/pnas.76.9.4350

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