Overview of Traditional Cloning

Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly and transformation. Learn more at https://www.neb.com/applications/clon...

0:00 - History of cloning
0:29 - Step 1: Identifying restriction sites
0:57 - Step 2: RE Digestion
1:27 - Step 3: Dephosphorylation and other end modifications
1:55 - Step 4: Ligation
2:00 - Step 5: Transformation
2:12 - Step 6:  Screen for desired clone
2:17 - Reagents for Fast cloning

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• #cloning
• #restriction_enzymes
• #synthetic_biology
• #dna