M-CSF Differentiated Monocyte-derived Macrophages | Protocol Preview
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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages - a 2 minute Preview of the Experimental Protocol
Xueting Jin, Howard S. Kruth
National Institutes of Health, Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute;
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages - a 2 minute Preview of the Experimental Protocol
Xueting Jin, Howard S. Kruth
National Institutes of Health, Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute;
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/05/10 منتشر شده
است.
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