Understanding Host-Induced Gene Silencing for Nematode Resistance Using RNAi Technology
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In this video, we explore
In this video, we explore the concept of host-induced gene silencing for nematode resistance using RNAi technology. Learn about the role of gene expression in plant-parasitic nematodes and how genetic engineering can be used to control nematode infestations. Discover the benefits of using host-induced gene silencing and RNAi technology in crop protection and how it can lead to more sustainable and eco-friendly pest control practices. Join us as we delve into the world of agriculture biotechnology and the latest innovations in pest control.
RNA interference
RNA interference or RNAi, has emerged as a very useful tool for gene silencing aimed at the functional analysis of different genes by suppressing their expression in a wide variety of organisms including plant parasitic nematodes.
In this strategy, the nematodes uptake double-stranded RNA or short interfering RNAs from the plants expressing these RNAs, which elicit a systemic RNAi response in nematodes.
The transgenic expression of double-stranded RNA targeting a specific nematode effector gene could be a handful to suppress the expression of that effector gene, which is crucial for the infection process.
Plant parasitic nematodes are obligate parasites of the plants and cannot be cultured on artificial media, hence we cannot make them uptake the double-stranded RNA directly from a medium or solution.
This limitation can be overcome by host-induced application of double-stranded RNA by expressing these RNAs in the host plant and feeding the nematodes on these plants.
We demonstrated it here in the form of a schematic diagram elaborating the mechanism of in-planta RNAi during molecular plant nematode interactions.
Double-stranded RNA produced in the transgenic plant can lead to RNA silencing of a specific gene in plant-parasitic nematodes. In the first step, the double-stranded RNA is processed by the plant DICER enzyme which cuts the double-stranded RNA into small interfering RNAs.
However, if longer double-stranded RNAs are ingested, the processing from double-stranded RNA to small interfering RNA can be performed by the nematode DICER as well.
But if the plant DICER does so, then, in the next step these small interfering RNA are taken up by the plant parasitic nematodes.
Once the small interfering RNA is ingested by the feeding nematodes on the transgenic plant, the small interfering RNA is recognized by the RNA-induced silencing complex or RISC machinery of the plant parasitic nematode, and its unwinding into sense and antisense strands takes place.
A proportion of the RISC complex loaded with the antisense strand interacts with the corresponding mRNA of the target gene in the nematode
And, as a result, the mRNA is cleaved by the RISC and subsequently degraded.
Moreover, the targeted mRNA can be made double-stranded after binding of the small interfering RNA, and this double-stranded RNA is then processed to produce additional small interfering RNAs, intensifying the initial silencing signal.
This is how host-induced gene silencing works in plant nematode interactions.
........................................
Host-induced gene silencing,
Nematode resistance,
RNAi technology,
Plant-parasitic nematodes,
Gene expression,
Genetic engineering,
Crop protection,
Agriculture,
Biotechnology,
Pest control,
Agriculture research,
Genetic modification,
Sustainable agriculture,
Agriculture innovation,
Agriculture technology,
.....................................
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Agri Knowledge Corridor: @agriknowledgecorridor
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RNA interference
RNA interference or RNAi, has emerged as a very useful tool for gene silencing aimed at the functional analysis of different genes by suppressing their expression in a wide variety of organisms including plant parasitic nematodes.
In this strategy, the nematodes uptake double-stranded RNA or short interfering RNAs from the plants expressing these RNAs, which elicit a systemic RNAi response in nematodes.
The transgenic expression of double-stranded RNA targeting a specific nematode effector gene could be a handful to suppress the expression of that effector gene, which is crucial for the infection process.
Plant parasitic nematodes are obligate parasites of the plants and cannot be cultured on artificial media, hence we cannot make them uptake the double-stranded RNA directly from a medium or solution.
This limitation can be overcome by host-induced application of double-stranded RNA by expressing these RNAs in the host plant and feeding the nematodes on these plants.
We demonstrated it here in the form of a schematic diagram elaborating the mechanism of in-planta RNAi during molecular plant nematode interactions.
Double-stranded RNA produced in the transgenic plant can lead to RNA silencing of a specific gene in plant-parasitic nematodes. In the first step, the double-stranded RNA is processed by the plant DICER enzyme which cuts the double-stranded RNA into small interfering RNAs.
However, if longer double-stranded RNAs are ingested, the processing from double-stranded RNA to small interfering RNA can be performed by the nematode DICER as well.
But if the plant DICER does so, then, in the next step these small interfering RNA are taken up by the plant parasitic nematodes.
Once the small interfering RNA is ingested by the feeding nematodes on the transgenic plant, the small interfering RNA is recognized by the RNA-induced silencing complex or RISC machinery of the plant parasitic nematode, and its unwinding into sense and antisense strands takes place.
A proportion of the RISC complex loaded with the antisense strand interacts with the corresponding mRNA of the target gene in the nematode
And, as a result, the mRNA is cleaved by the RISC and subsequently degraded.
Moreover, the targeted mRNA can be made double-stranded after binding of the small interfering RNA, and this double-stranded RNA is then processed to produce additional small interfering RNAs, intensifying the initial silencing signal.
This is how host-induced gene silencing works in plant nematode interactions.
........................................
Host-induced gene silencing,
Nematode resistance,
RNAi technology,
Plant-parasitic nematodes,
Gene expression,
Genetic engineering,
Crop protection,
Agriculture,
Biotechnology,
Pest control,
Agriculture research,
Genetic modification,
Sustainable agriculture,
Agriculture innovation,
Agriculture technology,
.....................................
See other informative videos on this channel
Concept of plant pathogens and parasites
Concept of Plant Pathogens, Parasites...
Detail of disease triangle and disease pyramid
Plant Disease Triangle, Disease Tetra...
Beneficial and harmful effects of microbes
Beneficial and Harmful aspects of mic...
Key features of plant pathogens and parasites
Key Features of Plant Pathogens, Viru...
Plant viruses, structure, nomenclature and transmission in plants
Plant viruses, structure, nomenclatur...
Plant virus life cycles
Plant virus life cycles
Prokaryotic plant pathogens
Prokaryotic plant pathogens, Bacteria...
Gram negative vs gram positive bacteria
Gram-negative vs gram-positive bacter...
Locomotion in bacteria, locomotors, locomotory organs in bacteria, flagella, pili
Locomotion in bacteria, locomotors, l...
Understanding the Importance of Nutrition in Bacteria: Autotrophs, Heterotrophs, and Nutrient Uptake
Understanding the Importance of Nutri...
...................................
Subscribe to this channel
Agri Knowledge Corridor: @agriknowledgecorridor
Like, share, and comment on the videos
Thanks
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در تاریخ 1402/01/22 منتشر شده
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