Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton l Protocol Preview
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Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton - a 2 minute Preview of the Experimental Protocol
Xiquan Gao, Robert C. Britt Jr., Libo Shan, Ping He
Texas A&M University, Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology; Texas A&M University, Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology;
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
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Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton - a 2 minute Preview of the Experimental Protocol
Xiquan Gao, Robert C. Britt Jr., Libo Shan, Ping He
Texas A&M University, Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology; Texas A&M University, Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology;
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/03/25 منتشر شده
است.
860
بـار بازدید شده