Identifying the N-Terminus of a Polypeptide

To prepare for a discussion of how peptide sequencing works, an understanding of the reactivity of the amino terminus of polypeptides is essential. This webcast describes two methods for identifying the N terminus of polypeptides. Both involve the use of an electrophilic, fluorescent "tag" molecule that reacts selectively with the N termini of polypeptides.

• #education
• #organic_chemistry
• #peptides
• #proteins
• #fluorescence
• #spectroscopy
• #hydrolysis