Edman Degradation

The Edman degradation takes advantage of unique nucleophilic reactivity of the N-terminal amino group to determine the sequence of a polypeptide from N-terminus to C-terminus, residue by residue.

00:00 Introduction
00:55 Cleavage and Cyclization by PIC
02:47 Mechanism of Cleavage by PIC
04:54 Summary

• #education
• #chemistry
• #organic_chemistry
• #biochemistry
• #amino_acids
• #polypeptides
• #edman_degradation
• #reaction_mechanisms