Western Blotting | Practical Applications

So far on the Demystifying Research channel, many aspects of the western blot have been discussed. This video expands on the practical applications such as diagnosing diseases to further expand western blot knowledge. This video dives into multiple diseases that are diagnosed using the Western Blot with an emphasis on Lyme disease. This video was made by McMaster University Students Nabeel Mubasher, Judah Izsak, Vipuja Sivayogarajah, Jennifer Nguyen in collaboration with the Demystifying Research McMaster Program. Copyright McMaster University 2022 Introduction: 0:00-0:11 What is Western Blotting: 0:12-0:48 Use of Western Blot: 0:49-1:15 Western Blot diagnoses: 1:16-1:25 HIV: 1:26-2:09 Tularemia: 2:10-2:45 Lyme Disease: 2:46-3:19 Diagnosing Lyme Disease: 3:20-3:34 ELISA: 3:25-4:08 Western Blotting Lyme Disease: 4:09-4:46 Overall: 4:47-5:09 Thank you + References: 5:10-5:20 References Cdc tularemia | faq about tularemia. (2019, February 21). emergency.cdc.gov/agent/tularemia/faq.asp Kim, B. (2017). Western blot techniques. In V. Espina (Ed.), Molecular Profiling: Methods and Protocols (pp. 133–139). Springer. doi.org/10.1007/978-1-4939-6990-6_9 Muccini, C., Galli, L., Spagnuolo, V., Poli, A., Rolla, S., Mastrangelo, A., Rinaldi, F., & Castagna, A. (2019). Brief report: Association between low hiv-1 dna and western blot reactivity to hiv-1 pol in chronically infected individuals. JAIDS Journal of Acquired Immune Deficiency Syndromes, 82(4), 373–376. doi.org/10.1097/QAI.0000000000002147 Papadopulos-Eleopulos, E., Turner, V. F., & Papadimitriou, J. M. (1993). Is a positive western blot proof of hiv infection? Bio/Technology, 11(6), 696–707. doi.org/10.1038/nbt0693-696 Rauer, S., Kayser, M., Neubert, U., Rasiah, C., & Vogt, A. (1995). Establishment of enzyme-linked immunosorbent assay using purified recombinant 83-kilodalton antigen of Borrelia burgdorferi sensu stricto and Borrelia afzelii for serodiagnosis of Lyme disease. Journal of Clinical Microbiology, 33(10), 2596–2600. doi.org/10.1128/jcm.33.10.2596-2600.1995 Russell, A. L. R., Dryden, M. S., Pinto, A. A., & Lovett, J. K. (2018). Lyme disease: Diagnosis and management. Practical Neurology, 18(6), 455–464. doi.org/10.1136/practneurol-2018-001998 Schmitt, P., Splettstösser, W., Porsch-Özcürümez, M., Finke, E.-J., & Grunow, R. (2005). A novel screening ELISA and a confirmatory Western blot useful for diagnosis and epidemiological studies of tularemia. Epidemiology and Infection, 133(4), 759–766. doi.org/10.1017/S0950268805003742 Steere, A. C., Green, J., Hutchinson, G. J., Rahn, D. W., Pachner, A. R., Schoen, R. T., Sigal, L. H., Taylor, E., & Malawista, S. E. (1987). Treatment of Lyme disease. Zentralblatt Für Bakteriologie, Mikrobiologie Und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, 263(3), 352–356. doi.org/10.1016/S0176-6724(87)80092-5 Western blot test: Uses, accuracy, procedure, cost & more. (2021, May 3). Healthline. www.healthline.com/health/hiv/western-blot-test Wright, W. F., Riedel, D. J., Talwani, R., & Gilliam, B. L. (2012). Diagnosis and management of Lyme disease. American Family Physician, 85(11), 1086–1093.

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