Western Blot: Introduction to Immunodetection

The western blot is a widely used research technique that detects proteins using antibodies. One important step of a western blot is called, ‘immunodetection’ which is the unique step which uses primary and secondary antibodies to detect the protein of interest. The video lists the materials and reagents needed for immunodetection and covers how to conduct it, using footage from a laboratory at The Research Institute of St. Joe's Hamilton/McMaster University.

This video was made by McMaster University students Aniqa Chaudhry, Brooke D'Mello, Ibrahim Ilahi, Kunwar Karim, Mubariz Maqsood, Adnan Moinuddin, and Julia Zhu in collaboration with the Demystifying Research McMaster Program.

We would like to also acknowledge the contributions of Dr. Takuma Isshiki, who provided guidance in the process. Finally, we would like to also thank Dr. Kjetil Ask and the Ask Lab for the additional guidance, insights and support.

Episode #1: Introduction to Western Blotting: Introduction to the Western Blot

Episode #2: SDS-PAGE: Western Blot: Introduction to SDS-PAGE

Episode #3: Wet Transfer: Western Blot: How to do a Wet Transfer

Episode #4: Electroblotting: Western Blot: Introduction to Electro...

Episode #5: Blocking: Western Blot: Introduction to Blocking

Episode #7: Results & Analysis: Western Blot: Result Interpretation

This video is for general and educational purposes only.

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References:

JG;, Gallagher S;Winston SE;Fuller SA;Hurrell. “Immunoblotting and Immunodetection.” Current Protocols in Molecular Biology, U.S. National Library of Medicine, https://pubmed.ncbi.nlm.nih.gov/18633....

“Western Blotting Immunodetection Techniques.” Bio, https://www.bio-rad.com/fr-ca/applica....

RM;, Hnasko TS;Hnasko. “The Western Blot.” Methods in Molecular Biology (Clifton, N.J.), U.S. National Library of Medicine, https://pubmed.ncbi.nlm.nih.gov/26160....