High Throughput Triacylglyceride Assay combined with High Fat Diet | Protocol Preview

JoVE (Journal of Visualized Experiments)
JoVE (Journal of Visualized Experiments)
124 بار بازدید - 2 سال پیش - Watch the Full Video at
Watch the Full Video at https://www.jove.com/v/56029/high-fat....

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster -  a 2 minute Preview of the Experimental Protocol

Soda Balla Diop, Ryan T. Birse, Rolf Bodmer
Sanford Burnham Prebys Medical Discovery Institute, Development, Aging and Regeneration Program;

This is a high fat diet feeding protocol to induce obesity in Drosophila, a model for understanding fundamental molecular mechanisms implicated in lipotoxicity. It also provides a high throughput triacylglyceride assay for measuring fat accumulation in Drosophila and potentially other (insect) models under various dietary, environmental, genetic or physiological conditions.

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2 سال پیش در تاریخ 1401/07/11 منتشر شده است.
124 بـار بازدید شده
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