Protein-RNA Complexes Detection by Gel Electrophoresis | Protocol Preview
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2 سال پیش
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Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes - a 2 minute Preview of the Experimental Protocol
Megan E. Dowdle, Susanne Blaser Imboden, Sookhee Park, Sean P. Ryder, Michael D. Sheets
University of Wisconsin School of Medicine and Public Health, Department of Biomolecular Chemistry; University of Massachusetts Medical School, Department of Biochemistry and Molecular Pharmacology;
Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
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Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes - a 2 minute Preview of the Experimental Protocol
Megan E. Dowdle, Susanne Blaser Imboden, Sookhee Park, Sean P. Ryder, Michael D. Sheets
University of Wisconsin School of Medicine and Public Health, Department of Biomolecular Chemistry; University of Massachusetts Medical School, Department of Biochemistry and Molecular Pharmacology;
Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/07/11 منتشر شده
است.
112
بـار بازدید شده