Frozen section in Histology/Frozen section biopsy/Frozen section preparation/STAR LABORATORY
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This video is about #frozen #section, in #Histology. Usage of #cryostat , or #freezing micro-tome #principle, #method, requirements and other explanations are clearly given. You can also study about cryostat or freezing #microtome.
The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. The technical name for this procedure is cryosection.
The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery.[1] The technical name for this procedure is cryosection.
The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing. While diagnosis can be rendered in many cases, fixed tissue processing is preferred in many conditions for more accurate diagnosis.
The key instrument for cryosection is the cryostat, which is essentially a microtome inside a freezer. The microtome can be compared to a very accurate "deli" slicer, capable of slicing sections as thin as 1 micrometre. The usual histology slice is cut at 5 to 10 micrometres. The surgical specimen is placed on a metal tissue disc which is then secured in a chuck and frozen rapidly to about –20 to –30 °C. The specimen is embedded in a gel like medium called OCT and consisting of poly ethylene glycol and polyvinyl alcohol; this compound is known by many names and when frozen has the same density as frozen tissue. At this temperature, most tissues become rock-hard. Usually a lower temperature is required for fat or lipid rich tissue. Each tissue has a preferred temperature for processing. Subsequently it is cut frozen with the microtome portion of the cryostat, the section is picked up on a glass slide and stained (usually with hematoxylin and eosin, the H&E stain). The preparation of the sample is much more rapid than with traditional histology technique (around 10 minutes vs 16 hours). However, the technical quality of the sections is much lower. The entire laboratory space can be less than 9-square-foot (0.84 m2), and minimal ventilation is required compared to a standard wax embedded specimen laboratory.
CRYOSTAT
A cryostat (from cryo meaning cold and stat meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium.[1] Hence it is usually assembled into a vessel, similar in construction to a vacuum flask or Dewar. Cryostats have numerous applications within science, engineering, and medicine.
Cryostat: A chamber that can maintain very low temperatures. Medical laboratories use a cryostat to preserve frozen tissue samples while a microtome, an extremely sharp cutting instrument mounted inside cryostats, slices the tissue into pieces thin enough to be observed under a microscope. The sliced piece must be so thin as to look nearly transparent. A pathologist, a laboratory doctor trained to identify evidence of disease in microscopic structures, then examines the slice to confirm or rule out the presence of a disease, such as cancer. Use of frozen tissue samples enables physicians to examine tissue and diagnose its condition more quickly than if the tissue had been preserved without freezing.
Microtomes may be used alone, without cryostats, to yield thin microscopic samples.
................................................................................................................
How do you prepare a frozen section?
How accurate is frozen section?
What is cryostat sectioning?
What do surgical pathologists do?
frozen section indications
frozen section biopsy accuracy
frozen section heart
principle of frozen section technique
excision with frozen section
frozen section bone
frozen section ovarian cyst
cap guidelines for frozen section turnaround time
frozen section biopsy accuracy
accuracy of frozen section pathology
fresh specimen vs frozen
h&e frozen section staining protocol
frozen section troubleshooting
oct tissue preparation
cryostat price
cryostat advantages and disadvantages
liquid nitrogen cryostat
cryostat brain slicing
care and maintenance of cryostat
freezing microtome
cryostat in pathology
cryostat slideshare
cryostat ppt
cryostat price
cryostat procedure
cryostat parts and functions
cryostat diagram
mohs cryostat
What is a cryostat microtome?
What is a freezing microtome?
How do you freeze tissue for cryostat sectioning?
How does a microtome work?
cryostat microtome parts
cryostat microtome price
cryostat in pathology
cryostat slideshare
cryostat ppt
cryostat sectioning
cryostat procedure
cryostat leica
The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. The technical name for this procedure is cryosection.
The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery.[1] The technical name for this procedure is cryosection.
The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing. While diagnosis can be rendered in many cases, fixed tissue processing is preferred in many conditions for more accurate diagnosis.
The key instrument for cryosection is the cryostat, which is essentially a microtome inside a freezer. The microtome can be compared to a very accurate "deli" slicer, capable of slicing sections as thin as 1 micrometre. The usual histology slice is cut at 5 to 10 micrometres. The surgical specimen is placed on a metal tissue disc which is then secured in a chuck and frozen rapidly to about –20 to –30 °C. The specimen is embedded in a gel like medium called OCT and consisting of poly ethylene glycol and polyvinyl alcohol; this compound is known by many names and when frozen has the same density as frozen tissue. At this temperature, most tissues become rock-hard. Usually a lower temperature is required for fat or lipid rich tissue. Each tissue has a preferred temperature for processing. Subsequently it is cut frozen with the microtome portion of the cryostat, the section is picked up on a glass slide and stained (usually with hematoxylin and eosin, the H&E stain). The preparation of the sample is much more rapid than with traditional histology technique (around 10 minutes vs 16 hours). However, the technical quality of the sections is much lower. The entire laboratory space can be less than 9-square-foot (0.84 m2), and minimal ventilation is required compared to a standard wax embedded specimen laboratory.
CRYOSTAT
A cryostat (from cryo meaning cold and stat meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium.[1] Hence it is usually assembled into a vessel, similar in construction to a vacuum flask or Dewar. Cryostats have numerous applications within science, engineering, and medicine.
Cryostat: A chamber that can maintain very low temperatures. Medical laboratories use a cryostat to preserve frozen tissue samples while a microtome, an extremely sharp cutting instrument mounted inside cryostats, slices the tissue into pieces thin enough to be observed under a microscope. The sliced piece must be so thin as to look nearly transparent. A pathologist, a laboratory doctor trained to identify evidence of disease in microscopic structures, then examines the slice to confirm or rule out the presence of a disease, such as cancer. Use of frozen tissue samples enables physicians to examine tissue and diagnose its condition more quickly than if the tissue had been preserved without freezing.
Microtomes may be used alone, without cryostats, to yield thin microscopic samples.
................................................................................................................
How do you prepare a frozen section?
How accurate is frozen section?
What is cryostat sectioning?
What do surgical pathologists do?
frozen section indications
frozen section biopsy accuracy
frozen section heart
principle of frozen section technique
excision with frozen section
frozen section bone
frozen section ovarian cyst
cap guidelines for frozen section turnaround time
frozen section biopsy accuracy
accuracy of frozen section pathology
fresh specimen vs frozen
h&e frozen section staining protocol
frozen section troubleshooting
oct tissue preparation
cryostat price
cryostat advantages and disadvantages
liquid nitrogen cryostat
cryostat brain slicing
care and maintenance of cryostat
freezing microtome
cryostat in pathology
cryostat slideshare
cryostat ppt
cryostat price
cryostat procedure
cryostat parts and functions
cryostat diagram
mohs cryostat
What is a cryostat microtome?
What is a freezing microtome?
How do you freeze tissue for cryostat sectioning?
How does a microtome work?
cryostat microtome parts
cryostat microtome price
cryostat in pathology
cryostat slideshare
cryostat ppt
cryostat sectioning
cryostat procedure
cryostat leica
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