Reverse Transcriptase PCR ll RT PCR II #csirnet#neetbiology #pcr #rtpcr
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The video contains a detailed
The video contains a detailed discussion of Reverse transcriptase PCR.
Reverse transcriptase polymerase chain reaction (RT-PCR) is a modified PCR-based amplification technique that uses RNA, rather than DNA, as the initial template.
Reverse transcriptase is an RNA-dependent DNA polymerase, catalyzing DNA synthesis using RNA as the template. The end product is known as complementary DNA (cDNA). cDNA is not subject to RNase degradation, making it more stable than RNA.
In RT-PCR, the starting RNA is subsequently degraded, dsDNA is produced, and PCR amplification proceeds in the usual manner.
#pcr #rtpcr #csirnet #bsc #neet
Reverse transcriptase polymerase chain reaction (RT-PCR) is a modified PCR-based amplification technique that uses RNA, rather than DNA, as the initial template.
Reverse transcriptase is an RNA-dependent DNA polymerase, catalyzing DNA synthesis using RNA as the template. The end product is known as complementary DNA (cDNA). cDNA is not subject to RNase degradation, making it more stable than RNA.
In RT-PCR, the starting RNA is subsequently degraded, dsDNA is produced, and PCR amplification proceeds in the usual manner.
#pcr #rtpcr #csirnet #bsc #neet
2 سال پیش
در تاریخ 1401/07/23 منتشر شده
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