Stem cell derived exosome production in stirred-tank bioreactors

Presented By: Jorge Escobar Aurélie Tacheny Speaker Biography: Jorge Escobar is a senior research scientist in the applications lab of Eppendorf, Inc., in Enfield, Connecticut, USA. He joined the company in 2019 and has worked on applied research focused on efficient expansion of adherent and non-adherent cells (e.g. stem cells, adult primary cells) in cell culture bioreactors. After his Ph.D. in Biomedical Engineering at the Polytechnic University of Valencia (UPV), he gained experience in the fields of cell therapy and regenerative medicine working as a researcher at the Center for Biomaterials and Tissue Engineering (UPV, Valencia, Spain), Institute of Biomaterial Sciences - Helmholtz-Zentrum Geesthacht (Berlin, Germany) and Institute for Regenerative Engineering (University of Connecticut Health, Connecticut, USA). Additionally, in 2017, he was promoted to Assistant Research Professor, Department of Chemical and Biomolecular Engineering, University of Connecticut. After a PhD in cell biology obtained at the uNamur, Belgium, and more than 10 years in the academic field, Aurélie Tacheny is now working for 6 years as Project Manager and Application Specialist for Eppendorf Application Technologies. Responsible for various cell culture-related projects, Aurélie and her team in the lab, use her expertise in this field to generate valuable experimental data and support current and future Eppendorf products and customers. Webinar: Stem cell derived exosome production in stirred-tank bioreactors Webinar Abstract: Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large number of proteins, lipids, messenger, and micro-RNAs. It was shown that they play a role in the mediation of intercellular communication, the modulation of immune-regulatory processes, tumor metabolism, and regenerative as well as degenerative processes. In recent years, there is increasing interest in the therapeutic potential of exosomes produced by Mesenchymal Stem Cells (MSCs). Stirred-tank bioreactors offer the possibility to tightly control and monitor the production of exosomes, as well as the scalability, to produce increasing amounts. However, the cultivation of stem cells in stirred-tank bioreactors requires profound knowledge and precise control of the process. In our talk, we will highlight the potential and benefits of stirred-tank bioreactors in the cultivation of stem cells for exosome production. We will share our knowledge on how we developed the process in our DASbox® Mini Parallel Bioreactor System and scaled-up the process to 1 L controlled by our newest parallel bioreactor control system, the SciVario® twin. Earn PACE Credits: 1. Make sure you’re a registered member of LabRoots (www.labroots.com/ms/webinar/stem-cell-derived-exos…) 2. Watch the webinar on YouTube or on the LabRoots Website (www.labroots.com/ms/webinar/stem-cell-derived-exos…) 3. Click Here to get your PACE credits (Expiration date – November 5, 2022): (www.labroots.com/credit/pace-credits/4935) LabRoots on Social: Facebook: www.facebook.com/LabRootsInc Twitter: twitter.com/LabRoots LinkedIn: www.linkedin.com/company/labroots Instagram: www.instagram.com/labrootsinc Pinterest: www.pinterest.com/labroots/ SnapChat: labroots_inc