Heat-Shock Transformation Protocol (for Bacteria)

Hey Friends,

the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.

Transformation, Conjugation and Transduction:
Transformation, Transduction and Conj...


References:
https://www.ncbi.nlm.nih.gov/pmc/arti...
https://www.ncbi.nlm.nih.gov/pmc/arti...


Thanks
Henrik


Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro

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