Determination of the Quality of a Sputum Specimen prior to culturing | Gram stain| Ideal sputum
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6 سال پیش
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|| Determination of the Quality
|| Determination of the Quality of a Sputum Specimen||
Prior to culturing a sputum specimen, a Gram stain should be performed to evaluate the quality of the specimen. One of most common criterion is currently used to determine if the specimen is contaminated with oral flora organisms, which would make the specimen unsuitable for culture. This criterion states that the sputum specimen should be rejected if more than 10 epithelial cells/low power field (LPF) and pus cells less than 25/low power field (LPF) are observed while specimen should be accepted for observation at oil immersion field and culturing if less than 10 epithelial cells/low power field (LPF) and pus cells greater than 25/low power field (LPF) are observed and this type sputum is also called ideal sputum.
The low power microscopic field of the video is representative of 20 microscopic fields that were reviewed on this Gram-stained preparation of sputum. This specimen would be considered acceptable for culture.
If the specimen falls in rejection criterion, the clinician should be contacted and a request should be made for a new specimen. It is important to communicate that culturing the specimen that was provided will not yield useful information about the possible pathogens from the lower respiratory tract.
If the specimen is determined to be a good quality lower respiratory tract specimen, continue to examine the slide under oil immersion magnification for bacteria, yeast, and polymorphonuclear cells (PMNs) also called pus cells and proceed with culturing the specimen.
From the video, Gram stain report:
Pus cells grater than 25/LPF
Epithelial cells less than10/LPF
Few Gram Negative bacilli seen
Prior to culturing a sputum specimen, a Gram stain should be performed to evaluate the quality of the specimen. One of most common criterion is currently used to determine if the specimen is contaminated with oral flora organisms, which would make the specimen unsuitable for culture. This criterion states that the sputum specimen should be rejected if more than 10 epithelial cells/low power field (LPF) and pus cells less than 25/low power field (LPF) are observed while specimen should be accepted for observation at oil immersion field and culturing if less than 10 epithelial cells/low power field (LPF) and pus cells greater than 25/low power field (LPF) are observed and this type sputum is also called ideal sputum.
The low power microscopic field of the video is representative of 20 microscopic fields that were reviewed on this Gram-stained preparation of sputum. This specimen would be considered acceptable for culture.
If the specimen falls in rejection criterion, the clinician should be contacted and a request should be made for a new specimen. It is important to communicate that culturing the specimen that was provided will not yield useful information about the possible pathogens from the lower respiratory tract.
If the specimen is determined to be a good quality lower respiratory tract specimen, continue to examine the slide under oil immersion magnification for bacteria, yeast, and polymorphonuclear cells (PMNs) also called pus cells and proceed with culturing the specimen.
From the video, Gram stain report:
Pus cells grater than 25/LPF
Epithelial cells less than10/LPF
Few Gram Negative bacilli seen
6 سال پیش
در تاریخ 1397/11/28 منتشر شده
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