Fluorescence Polarization Anisotropy | A Detailed Lesson | Score high in exam. | Lecture Notes
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Readymade answer-paper for your exam. Lecture notes. Study material.
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real exam. question-answer:
What does Fluorescence Anisotropy actually imply?
What is Fluorescence Anisotropy technique based on?
What is the origin of Anisotropy?
Where is Fluorescence Polarization relevant?
Why using polarization, not the ordinary fluorescence experiment?
Why is Fluorescence Polarization technique a better alternative?
Why is Anisotropy preferred?
What does it have to do with molecular rigidity?
What does it have to do with rotational mobility?
When plane polarized light excites a small fluorescent molecule bound to a larger one, what would be the output?
Why does the emission light have longer wavelength than that of the excitation light? Why does the emission light have less energy than that of the excitation light?
What type of instrument should we use in Fluorescence Polarization studies?
If your samples are exhibiting non-zero Anisotropy, what does that imply?
How is rotational diffusion related to Anisotropy?
What is the physical basis of Fluorescence Polarization assays?
Why measuring/comparing in vertical and horizontal directions?
Why do relaxations occur?
What is Fluorescence Anisotropy sensitive to?
What is polarized light used for in FPA technique?
What are the advantages of FPA technique?
What are the roles of the instrument components?
What is the reason additional separation steps are not required?
What is dual emission detection mode?
DISCLAIMER: The facts and information provided in this video are meant only for a sole purpose of knowledge, guidance and helpful suggestions, absolutely free of charges. I am not endorsing any product, facility or service in any way. The creator and owner of this video disclaims any liability in connection with the use of these information and/or decision-making. The creator and owner of this video disclaims responsibility for any decision made by individuals that may lead to eventual fallacy. The viewers must make their own decision through their own judgement, perspective and needs. The information provided is subject to change without notice.
energy states, luminescence, Frank-Condon Principle, absorption, excitation, emission, polarization, anisotropy, fluorometer, electronic transition, Jablonski, electromagnetic radiation, Planck’s Law, dipole moment, stokes shift, mirror image rule, fluorescence, phosphorescence, internal conversion, vibrational relaxation, rotational diffusion, intersystem crossing, lifetime, spin states, quantum yield, fluorophore, atomic structure, spectroscopy, biomolecular interaction, binding assay technology, chemical biology, steady-state, time-resolved, biophysical research, clinical diagnostics, high-throughput screening, exam question answer, physical chemistry, thermo fisher, sciencedirect, quantum mechanics,
#fluorescence, #stokes, #polarizationanisotropy, #fluorophorechromophore, #phosphorescence, #absorptionemission, #electronictransition, #atomicspectroscopy, #luminescence, #spectroscopy, #absorptionemission, #fluorescence, #fluorescencepolarizationanisotropy, #electronictransition, #opticalphysics,
Readymade answer-paper for your exam. Lecture notes. Study material.
Be brilliant in exam. Easy learning. Grown-up late beginners’ choice.
I have over 200 videos on elaborate lessons in Optical Physics and Physical Chemistry in my channel that you can consult as per need.
My channel and my videos are made for knowledge-seeking grown-up beginner viewers and education-seeking college/university students. Please note that, I do not try to impress by high-tech tricks and gimmicks. My low-tech productions are fine for devoted learners. Att: If you already "know it all", my videos are not for you.
check out https://www.emeraldcloudlab.com/instr...
real exam. question-answer:
What does Fluorescence Anisotropy actually imply?
What is Fluorescence Anisotropy technique based on?
What is the origin of Anisotropy?
Where is Fluorescence Polarization relevant?
Why using polarization, not the ordinary fluorescence experiment?
Why is Fluorescence Polarization technique a better alternative?
Why is Anisotropy preferred?
What does it have to do with molecular rigidity?
What does it have to do with rotational mobility?
When plane polarized light excites a small fluorescent molecule bound to a larger one, what would be the output?
Why does the emission light have longer wavelength than that of the excitation light? Why does the emission light have less energy than that of the excitation light?
What type of instrument should we use in Fluorescence Polarization studies?
If your samples are exhibiting non-zero Anisotropy, what does that imply?
How is rotational diffusion related to Anisotropy?
What is the physical basis of Fluorescence Polarization assays?
Why measuring/comparing in vertical and horizontal directions?
Why do relaxations occur?
What is Fluorescence Anisotropy sensitive to?
What is polarized light used for in FPA technique?
What are the advantages of FPA technique?
What are the roles of the instrument components?
What is the reason additional separation steps are not required?
What is dual emission detection mode?
DISCLAIMER: The facts and information provided in this video are meant only for a sole purpose of knowledge, guidance and helpful suggestions, absolutely free of charges. I am not endorsing any product, facility or service in any way. The creator and owner of this video disclaims any liability in connection with the use of these information and/or decision-making. The creator and owner of this video disclaims responsibility for any decision made by individuals that may lead to eventual fallacy. The viewers must make their own decision through their own judgement, perspective and needs. The information provided is subject to change without notice.
energy states, luminescence, Frank-Condon Principle, absorption, excitation, emission, polarization, anisotropy, fluorometer, electronic transition, Jablonski, electromagnetic radiation, Planck’s Law, dipole moment, stokes shift, mirror image rule, fluorescence, phosphorescence, internal conversion, vibrational relaxation, rotational diffusion, intersystem crossing, lifetime, spin states, quantum yield, fluorophore, atomic structure, spectroscopy, biomolecular interaction, binding assay technology, chemical biology, steady-state, time-resolved, biophysical research, clinical diagnostics, high-throughput screening, exam question answer, physical chemistry, thermo fisher, sciencedirect, quantum mechanics,
#fluorescence, #stokes, #polarizationanisotropy, #fluorophorechromophore, #phosphorescence, #absorptionemission, #electronictransition, #atomicspectroscopy, #luminescence, #spectroscopy, #absorptionemission, #fluorescence, #fluorescencepolarizationanisotropy, #electronictransition, #opticalphysics,
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