Microscopy: Resolution in Microscopy (Jeff Lichtman)

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This lecture describes the diffraction of light, a key principle in image formation and a factor that limits the resolution of a conventional light microscope. The behavior of light traveling through an objective is described along with the concept of numerical aperture.  The "point spread function" (or PSF) and Nyquist sampling are explained, which are critical concepts for understanding image resolution and detection of images.

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