Titrating for flow cytometry experiments

Titration of antibodies is one of the most critical aspects of a flow cytometry experiment. In this video, I give a brief overview of the basics of setting up a titration for a flow cytometry experiment. My concentration will be different from what you do in the lab- so make sure you have enough volume in your first sample in order to remove the necessary volume for the dilution series AND to add the appropriate volume to your samples. For example, if you are doing a 1 in 2 dilution series and adding 100 uL to each sample, you will need to start with a minimum of 200 uL in your first tube!

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