CRISPR/Cas9 Gene Knockouts Generation in Mammalian Cells | Protocol Preview
1 هزار بار بازدید -
2 سال پیش
-
Watch the Full Video at
Watch the Full Video at https://www.jove.com/v/55903/selectio....
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells - a 2 minute Preview of the Experimental Protocol
Erin L. Sternburg, Kristen C. Dias, Fedor V. Karginov
University of California, Riverside, Cell Biology and Neuroscience;
Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells - a 2 minute Preview of the Experimental Protocol
Erin L. Sternburg, Kristen C. Dias, Fedor V. Karginov
University of California, Riverside, Cell Biology and Neuroscience;
Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/07/11 منتشر شده
است.
1,036
بـار بازدید شده