Application of DNA–DNA hybridization Urdu/Hindi by Mushtaq Saqib
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3 سال پیش
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DNA hybridization provides an extremely
DNA hybridization provides an extremely powerful tool in molecular biology. Hybridization allows the identification and cloning of specific genes, analysis of levels of mRNA in cells, analysis of the copy number of sequences in the genome, and DNA fingerprinting, among other applications.
DNA hybridization
DNA hybridization was the first DNA-based technique proposed for the molecular discrimination of Eimeria parasites (Shirley, 1994b). A typical protocol consisted of genomic DNA digestion with different restriction enzymes, separation through agarose gel electrophoresis, blotting and hybridization with DNA probes composed of repetitive regions. The final result was a DNA fingerprinting comprising multiple band profiles. Similarly to enzyme variation detection, this approach also required large numbers of parasites and was highly time demanding. Also, the method was inherently unable to deal with mixed samples, since overlapping band profiles are not informative.
F ECOTOXICITY ASSESSMENT
DNA hybridization arrays can serve as high-throughput tools to assess chemical contaminants in soil (Fredrickson et al., 2001). By spotting sequences of microbial genes that are induced by specific soil contaminants by resident soil microorganisms, the expression profiles of the aggregate soil microorganisms can be used to detect and classify the contamination. While the analysis hardware and software is directly transferable from the more traditional glass slide microarray applications, questions remain about the environmental relevance of microbiological mRNA level responses. The increasing quantities of genomic information available on environmental organisms makes the development of this technology more feasible.
Hi am Mushtaq Saqib and you are watching #Botany zoom the one and only destination for smart learning. we provide free lecture related to Biology, botany, zoology, biochemistry, pharmacology. we also upload general awareness content. you can get huge information about plants through my Facebook page with name of (Botany zoom) . link about this page is here Facebook: Botany-Zoom- you can also contact with me through my personal Facebook id via this link Facebook: malik.mushta.. also via phone no / WhatsApp 03013813262
DNA hybridization
DNA hybridization was the first DNA-based technique proposed for the molecular discrimination of Eimeria parasites (Shirley, 1994b). A typical protocol consisted of genomic DNA digestion with different restriction enzymes, separation through agarose gel electrophoresis, blotting and hybridization with DNA probes composed of repetitive regions. The final result was a DNA fingerprinting comprising multiple band profiles. Similarly to enzyme variation detection, this approach also required large numbers of parasites and was highly time demanding. Also, the method was inherently unable to deal with mixed samples, since overlapping band profiles are not informative.
F ECOTOXICITY ASSESSMENT
DNA hybridization arrays can serve as high-throughput tools to assess chemical contaminants in soil (Fredrickson et al., 2001). By spotting sequences of microbial genes that are induced by specific soil contaminants by resident soil microorganisms, the expression profiles of the aggregate soil microorganisms can be used to detect and classify the contamination. While the analysis hardware and software is directly transferable from the more traditional glass slide microarray applications, questions remain about the environmental relevance of microbiological mRNA level responses. The increasing quantities of genomic information available on environmental organisms makes the development of this technology more feasible.
Hi am Mushtaq Saqib and you are watching #Botany zoom the one and only destination for smart learning. we provide free lecture related to Biology, botany, zoology, biochemistry, pharmacology. we also upload general awareness content. you can get huge information about plants through my Facebook page with name of (Botany zoom) . link about this page is here Facebook: Botany-Zoom- you can also contact with me through my personal Facebook id via this link Facebook: malik.mushta.. also via phone no / WhatsApp 03013813262
3 سال پیش
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