How to select LCMS interfaces: working principle of ESI, APCI and DUIS Interface

How to select LCMS interfaces: working principle of ESI, APCI and DUIS Interface was explained in this video. The commonly used ionization interfaces for LCMS are electrospray ionization interface, in short ESI interface, and atmospheric pressure chemical ionization interface, which is also known as APCI interface. In addition, DUIS interface was also introduced, DUIS interface allows ionization of analyte using ESI and APCI techniques concurrently. DUIS interface can produce very effective ionization when simultaneous analysis of analyte that can be only ionized by ESI interface and analyte that can only be ionized by APCI interface is required. Some of the common application that uses ESI interface for analyses are screening for residual pesticides, analysis of veterinary drugs and analysis of porcine peptides for halal application. Some of the common application that uses APCI interface for analyses are analysis of nitrosamine, analysis of antioxidants such as chlorophyll and carotenoids and analysis of phenols and its derivatives. Comparation of analysis results from ESI, APCI and DUIS ionization, it showed that ESI interface is good for analysis of water-soluble vitamin while APCI interface is good for analysis of fat-soluble vitamin. Good signal intensity was observed using DUIS interface for all target analytes, vitamin B1, vitamin B2 and vitamin D2. With DUIS interface, analysis of water soluble and fat-soluble vitamins can be done simultaneously. Reference data articles can be found at: Simultaneous Determination of Pesticide Residues in Vegetable Extract by LC/MS/MS [LCMS™-8050] https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/applications/application_note/13748/jpo120009.pdf Simultaneous determination of 130 veterinary drugs in pork using ultra performance liquid chromatograph-tandem mass spectrometry https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/white_papers/13813/hko119041.pdf Detection and Differentiation of Bovine and Porcine Gelatins in Food and Pharmaceutical Products By LC/MS/MS Method https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/applications/application_note/10806/apo118005.pdf A Direct Method for the Determination of 13 Nitrosamines in Water by LCMS-8045 System https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/applications/application_note/16649/an_06-saip-lc-048-en.pdf Analysis of photosynthetic pigments and quinones in chloroplast using LC-MS https://www.an.shimadzu.co.jp/aplnotes/lcms/an_c20.pdf A High Sensitivity Method for Quantitative Determination of Ten Phenols in Surface Water on LC/MS/MS with APCI Interface https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/applications/application_note/13866/apo117021.pdf Dual Ion Source DUIS-2020 for Simultaneous ESI and APCI Measurement https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/applications/application_note/12456/jpo212010.pdf #Shimadzu_LCMS #LCMS_Interface #Working_prinicple_of_LCMS_inteface #ESI #APCI #DUIS

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