Baculovirus expression Vectors: AcMNPV @Dr.DNA-Primer
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2 سال پیش
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The specific baculovirus that has
The specific baculovirus that has been used extensively is Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) AcMNPV: Autographa californica Multip...
The production of recombinant baculovirus expression vectors involves the following steps
1. A gene of interest is inserted into the MCS of Transfer vector and propagated in E. coli.
2. Next, insect cells in culture are cotransfected with AcMNPV DNA and the Transfer vector carrying the cloned gene.
3. Selection of recombinant AcMNPV genome virus particle
The production of recombinant baculovirus expression vectors involves the following steps
1. A gene of interest is inserted into the MCS of Transfer vector and propagated in E. coli.
2. Next, insect cells in culture are cotransfected with AcMNPV DNA and the Transfer vector carrying the cloned gene.
3. Selection of recombinant AcMNPV genome virus particle
2 سال پیش
در تاریخ 1401/07/16 منتشر شده
است.
965
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