DLC Test | WBC Differential Count | Differential Leukocyte Count
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5 سال پیش
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This video demonstrates everything you
This video demonstrates everything you need to know about DLC Test Procedure i.e. Differential Leukocyte Count ( DLC Test Procedure ). It is also known as WBC differential count, DLC count, WBC differential count Test, etc. In Hindi we call it as सफेद रक्त कोशिका जांच, #DLCtest kaise karte hai?
Leishman’s Stain solution : https://amzn.to/2lBmaNB
For detailed information visit: https://www.ebiologylab.com/experimen...
Before starting with a practical DLC test, we will look into different types of leukocytes.
There are two major categories of #leukocytes.
1.) #Granulocytes:
Neutrophils: Neutrophils are a common type of white blood cell (WBCs). They are accountable for destroying germs in injured or infected tissue.
Eosinophils: Eosinophils treat infections caused by parasites and also control the immune system's response to allergic reactions.
Basophils: These are the least common type of white blood cells. Their function is unknown, but they may play a role in allergic reactions.
2.) #Agranulocytes:
Lymphocytes: There are three types of lymphocytes.
@ B lymphocytes: produce antibodies to attack bacteria, viruses, and other foreign invaders.
@ T lymphocytes: help to identify cells that require an immune response.
@ Natural killer cells: destroy cancer cells and viruses.
Monocytes: These cells are similar to neutrophils. They destroy bacteria, those causing chronic infection and also repair damaged tissues.
The function of each type of WBC is specific and their number is also different per 100 counts.
#Neutrophils: 40–60 percent
#Eosinophils: 1–4 percent
#Basophils: 0.5–1.0 percent
#Lymphocytes: 20–40 percent
#Monocytes: 2–8 percent
Different physiological and clinical conditions cause variation in different WBC count ( DLC Test )
Hence the deviation from the normal count of different WBCs per 100 WBC helps to detect is Eosinophilia, tuberculosis, malaria and other diseases
This type of counting is known as differential count.
Let's begin with our practical DLC test:
To stain WBCs, we are going to use Leishman stain that contains both acidic and basic dyes
Blood Film Preparation: for DLC test
Sterilize your fingertip with rectified spirit and pri.k it with needle
Place a drop of blood at one end of the clean slide.
Use another slide as a spreader to spread that blood drop into a thin smear.
To achieve a thin layer of blood, maintain a 45-degree angle between spreader and blood slide.
You will get uniform blood film in a tongue shape nature.
Till the slides get dried, prepare the staining rack.
Put the air-dried slide on the staining rack and add sufficient amount of Stain with the help of dropper on the blood film.
Wait for 1 to 2 minutes to allow proper fixing.
After 2 minutes add the double amount of distilled water or Leishman buffer over the film carefully.
After 10 minutes wash the slide under running water.
Let the slide get dried in the air.
Start the counting from one end of the film to another and note the different types of leukocytes from each field under the Microscope.
The counting is continued till the total number of leukocytes i.e. WBCs become hundred.
Previous video: WHITE BLOOD CELLS
White Blood Cell Identification | WBC...
Leishman’s Stain solution : https://amzn.to/2lBmaNB
For detailed information visit: https://www.ebiologylab.com/experimen...
Before starting with a practical DLC test, we will look into different types of leukocytes.
There are two major categories of #leukocytes.
1.) #Granulocytes:
Neutrophils: Neutrophils are a common type of white blood cell (WBCs). They are accountable for destroying germs in injured or infected tissue.
Eosinophils: Eosinophils treat infections caused by parasites and also control the immune system's response to allergic reactions.
Basophils: These are the least common type of white blood cells. Their function is unknown, but they may play a role in allergic reactions.
2.) #Agranulocytes:
Lymphocytes: There are three types of lymphocytes.
@ B lymphocytes: produce antibodies to attack bacteria, viruses, and other foreign invaders.
@ T lymphocytes: help to identify cells that require an immune response.
@ Natural killer cells: destroy cancer cells and viruses.
Monocytes: These cells are similar to neutrophils. They destroy bacteria, those causing chronic infection and also repair damaged tissues.
The function of each type of WBC is specific and their number is also different per 100 counts.
#Neutrophils: 40–60 percent
#Eosinophils: 1–4 percent
#Basophils: 0.5–1.0 percent
#Lymphocytes: 20–40 percent
#Monocytes: 2–8 percent
Different physiological and clinical conditions cause variation in different WBC count ( DLC Test )
Hence the deviation from the normal count of different WBCs per 100 WBC helps to detect is Eosinophilia, tuberculosis, malaria and other diseases
This type of counting is known as differential count.
Let's begin with our practical DLC test:
To stain WBCs, we are going to use Leishman stain that contains both acidic and basic dyes
Blood Film Preparation: for DLC test
Sterilize your fingertip with rectified spirit and pri.k it with needle
Place a drop of blood at one end of the clean slide.
Use another slide as a spreader to spread that blood drop into a thin smear.
To achieve a thin layer of blood, maintain a 45-degree angle between spreader and blood slide.
You will get uniform blood film in a tongue shape nature.
Till the slides get dried, prepare the staining rack.
Put the air-dried slide on the staining rack and add sufficient amount of Stain with the help of dropper on the blood film.
Wait for 1 to 2 minutes to allow proper fixing.
After 2 minutes add the double amount of distilled water or Leishman buffer over the film carefully.
After 10 minutes wash the slide under running water.
Let the slide get dried in the air.
Start the counting from one end of the film to another and note the different types of leukocytes from each field under the Microscope.
The counting is continued till the total number of leukocytes i.e. WBCs become hundred.
Previous video: WHITE BLOOD CELLS
White Blood Cell Identification | WBC...
5 سال پیش
در تاریخ 1397/12/04 منتشر شده
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