Cancer Cells Death Evaluation using Probiotics in 3D Cultures | Protocol Preview
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Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells - a 2 minute Preview of the Experimental Protocol
Jina Lee, Joo-Eun Lee, Seil Kim, Dukjin Kang, Hee Min Yoo
Korea Research Institute of Standards and Science (KRISS), Microbiological Analysis Team, Group for Biometrology; Chungnam National University (CNU), College of Pharmacy; Korea Research Institute of Bioscience and Biotechnology (KRIBB), Stem Cell Research Center; Korea Research Institute of Chemical Technology (KRICT), Convergent Research Center for Emerging Virus Infection; University of Science & Technology (UST), Department of Bio-Analysis Science;
Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.
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Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells - a 2 minute Preview of the Experimental Protocol
Jina Lee, Joo-Eun Lee, Seil Kim, Dukjin Kang, Hee Min Yoo
Korea Research Institute of Standards and Science (KRISS), Microbiological Analysis Team, Group for Biometrology; Chungnam National University (CNU), College of Pharmacy; Korea Research Institute of Bioscience and Biotechnology (KRIBB), Stem Cell Research Center; Korea Research Institute of Chemical Technology (KRICT), Convergent Research Center for Emerging Virus Infection; University of Science & Technology (UST), Department of Bio-Analysis Science;
Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/12/25 منتشر شده
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