Clinical Chemistry 1 Immunoassays

An overview of immunoassays in clinical chemistry, based on Larson's Clinical Chemistry, chapter 4 on immunoassays. Basic principles of immunoassays, heterogenous vs homogenous, competitive vs non-competitive, and various specific immunoassays. Calibration, quality control and the hook effect.

Videos links: Enzyme immunoassay (EIA) to detect an...
How Electrochemiluminescence (ECL) Works
Fluorescence Polarization
PPT Handout: https://drive.google.com/file/d/16kO8...

0:00 Introduction
0:21 Antibodies, Antigens, and Analytes
2:28 Basics of Immunochemical Reactions
5:39 Antibodies Are Immunoglobulins
6:54 Affinity and Avidity
9:04 Heterogeneous Immunoassays
10:14 Homogeneous Immunoassays
15:18 Noncompetitive Immunoassays
17:04 Immunoassay Labels
19:25 Enzyme-Linked Immunosorbent Assay
24:28 Enzyme-Multiplied Immunoassay Technology
27:37 Fluorescence Polarization
30:47 Microparticle Enzyme Immunoassay
32:42 Chemiluminescent Microparticle
35:37 Cloned Enzyme Donor Immunoassay
37:23 Particle Methods
38:05 Immunodiffusion
40:27 Immunoelectrophoresis: Western blot
42:12 Light-Scattering Methods
43:21 Factors Affecting Immunoassay Performance
45:43 Hook Effect