Target Binding and CDC Induction Between Therapeutic Antibodies | Protocol Preview
270 بار بازدید -
2 سال پیش
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In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis - a 2 minute Preview of the Experimental Protocol
Nohemi Salinas-Jazmín, Edith González-González, Luz X. Vásquez-Bochm, Sonia M. Pérez-Tapia, Marco A. Velasco-Velázquez
National Polytechnic Institute (IPN), University of Mexico (UNAM), Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences; National Autonomous University of Mexico (UNAM), School of Chemistry; National Autonomous University of Mexico (UNAM), Graduate Program in Chemical Sciences; National Polytechnic Institute (IPN), Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences; National Polytechnic Institute (IPN), Department of Immunology, National Scool of Biological Sciences; National Autonomous University of Mexico (UNAM), Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine;
This protocol describes the in vitro comparison of two key functional characteristics of rituximab: target binding and complement-dependent cytotoxicity (CDC) induction. The methods were employed for a side-to-side comparison between reference rituximab and a rituximab biosimilar. These assays can be employed during biosimilar development or as a quality control in their production.
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In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis - a 2 minute Preview of the Experimental Protocol
Nohemi Salinas-Jazmín, Edith González-González, Luz X. Vásquez-Bochm, Sonia M. Pérez-Tapia, Marco A. Velasco-Velázquez
National Polytechnic Institute (IPN), University of Mexico (UNAM), Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences; National Autonomous University of Mexico (UNAM), School of Chemistry; National Autonomous University of Mexico (UNAM), Graduate Program in Chemical Sciences; National Polytechnic Institute (IPN), Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences; National Polytechnic Institute (IPN), Department of Immunology, National Scool of Biological Sciences; National Autonomous University of Mexico (UNAM), Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine;
This protocol describes the in vitro comparison of two key functional characteristics of rituximab: target binding and complement-dependent cytotoxicity (CDC) induction. The methods were employed for a side-to-side comparison between reference rituximab and a rituximab biosimilar. These assays can be employed during biosimilar development or as a quality control in their production.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/05/12 منتشر شده
است.
270
بـار بازدید شده